The Masters: Defending champion Johnson misses cut along with Koepka, Garcia and McIlroy

Johnson, who won the Masters for the first time in November, shot a three-over 75 on the second day to be five over overall and miss the cut by two strokes.

World number one Johnson made three bogeys on his final four holes to miss out, having previously impressed at Augusta, with top-10 finishes in his past five appearances.

He made four birdies on the front nine but also had two bogeys and a double bogey on the fifth.

Johnson becomes the third Masters champion in the past 15 years to miss the cut.

"Six three-putts in two rounds, you just can't do that," Johnson said. "Obviously didn't drive it great but drove it good enough.

"I never was really too bad out of position, just the three-putts killed me. You take all the three-putts away, I'm one under. That was kind of the difference."

The 36-year-old was joined by four-time major winner Koepka with a similar round to miss the cut, along with 2017 Masters winner Garcia, who shot an even round of 72 to finish at four over.

Four-time major winner McIlroy (74) and two-time Masters runner-up Lee Westwood (71) also failed to make the cut, with both unable to recover after poor first days at Augusta.

At the other end of the leaderboard, Rose sits one shot clear of Will Zalatoris and Brian Harman after a second-round 72, having led by four shots on the opening day.

Rose bogeyed four of his first seven holes on Friday, before steadying with three birdies on the back nine to salvage his lead.

"I think it was just a classic day at Augusta National when you're just slightly off," Rose said.

"You can be a foot or two out on certain occasions and you end up struggling. I think maybe off the back of yesterday, it starts to feel pretty different pretty quickly. But I told myself going up the eighth hole, you're leading the Masters, your frame of reference is a little bit different to yesterday. Four ahead is something, but you're still leading so like just enjoy it and keep going."

On his Masters debut, 24-year-old Zalatoris moved up the leaderboard swiftly, aided by five birdies on his back nine, including three straight to finish his round as he carded a four-under 68.

Harman also moved into contention after three birdies on his final six holes to finish with a three-under 69.

Australian Marc Leishman and last week's Texas Open winner Jordan Spieth are tied for fourth after both enjoyed strong rounds on Friday.

Bernd Wiesberger and Tony Finau both shot 66s on Friday to storm up the leaderboard into joint sixth alongside four other players, including world number two Justin Thomas.